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Morphological and electron microscopic effects of mitomycin C inhalation in experimentally induced laryngeal fibrosis in dogs

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Abstract

Purpose

This study was carried out to investigate the morphological and ultrastructural effects of mitomycin C in the inhalational form on induced laryngeal tissue injuries and fibrosis.

Materials and methods

This study was carried out on 17 cross-breed clinically healthy dogs. Three dogs were included in a normal control group. The rest of the 14 dogs were subjected to induction of laryngeal fibrosis and assigned randomly to two equal groups: one treated with inhalational mitomycin C and the other was an untreated control group. Tissue biopsies were studied after H&E and Masson’s trichrome stains, whereas other pieces were examined by electron microscopy.

Results

Epithelial ulceration with marked inflammatory infiltration, edema, and congestion was evident in all experimented dogs. There was a significant difference in epithelial hyperplasia between the treated and the untreated groups; it was markedly reduced in the treated group. The untreated group showed extensive well-organized fibrosis that was significantly increased compared with the other groups. Results were confirmed by electron microscopy.

Conclusion

Inhalation of mitomycin C has a potent antifibrotic effect through decreasing the activity of fibroblast evident by the decrease in both the size of fibroblast and the density of the intrafibroblast endoplasmic reticulum.

References

  1. Smith ME, Elstad M. Mitomycin C and the endoscopic treatment of laryngotracheal stenosis: are two applications better than one? Laryngoscope 2009; 119: 272–283.

    Article  CAS  Google Scholar 

  2. Roh JL, Yoon YH. Prevention of anterior glottic stenosis after bilateral vocal fold stripping with mitomycin C. Arch Otolaryngol Head Neck Surg 2005; 131: 690–695.

    Article  Google Scholar 

  3. Spector JE, Huang S, Werkhaven JA, Sanders D, Spector NC, Reinisch L. Prevention of anterior glottic restenosis in a canine model with topical mitomycin-C. Ann Otol Rhinol Laryngol 2001; 110: 1007–1010.

    Article  CAS  Google Scholar 

  4. Fattah HA, Hamza A, Gaafar A, Hamza M, Mourad Z. Inhalation mitomycin-C in the management of laryngeal fibrosis: rationale, benefits and pitfalls. Int Congress Ser 2003; 1240: 831–837.

    Article  Google Scholar 

  5. Cortés de Miguel S, Cabeza Barrera J, Gallardo Medina M, Cassini Gmez De Cdiz LF, Salmern-Garca A, Rodrguez Lucas F. Topical endotracheal mitomycin C as a complementary treatment for endoscopic treatment of recurrent laryngotracheal stenosis. Farm Hosp 2011; 35:32–35.

    Article  Google Scholar 

  6. Garrett CG, Soto J, Riddick J, Billante CR, Reinisch L. Effect of mitomycin-C on vocal fold healing in a canine model. Ann Otol Rhinol Laryngol 2001; 110: 25–30.

    Article  CAS  Google Scholar 

  7. Fang R, Sun J, Wan G, Sun D. Comparison between mitomycin C and chitosan for prevention of anterior glottic steno after CO 2 laser cordectomy in dogs. Laryngoscope 2007; 117: 2057–2062.

    Article  Google Scholar 

  8. Eliashar R, Davros W, Eliachar I, Gramlich T, Esclamado R, Moffett K, Strome M. Evaluating laryngotracheal stenosis in a canine model with virtual bronchoscopy. Ann Otol Rhinol Laryngol 2000; 109: 906–912.

    Article  CAS  Google Scholar 

  9. Karmel M. Rethinking mitomycin C. Tips from glaucoma and refractive surgeons on ways to get the most out while minimizing complications. 2013. Available at: http://www.aao.org/publications/eyenet/200503/feature.cfm. [Last accessed date on 2013 Feb 4].

  10. Spector JE, Werkhaven JA, Spector NC, Huang S, Page RN, Baranowski B, et al. Preservation of function and histologic appearance in the injured glottis with topical mitomycin-C. Laryngoscope 1999; 109:1125–1129.

    Article  CAS  Google Scholar 

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Correspondence to Mohamed T. El Tabbakh MD.

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El Tabbakh, M.T., Zagholol, M.M.H.M., Hinnis, A.R. et al. Morphological and electron microscopic effects of mitomycin C inhalation in experimentally induced laryngeal fibrosis in dogs. Egypt J Otolaryngol 30, 126–131 (2014). https://doi.org/10.4103/1012-5574.133214

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  • DOI: https://doi.org/10.4103/1012-5574.133214

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