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The Egyptian Journal of Otolaryngology
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Does angiogenesis have a prognostic value in squamous cell carcinoma of the larynx?

The Egyptian Journal of Otolaryngology volume 31, pages 92–97 (2015)Cite this article

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Abstract

Introduction

Angiogenesis marks a tumour’s transition to a systemic disease. It is a useful prognostic and predictive factor; yet, its relationship to prognosis in squamous cell carcinoma of the larynx is controversial.

Aim of this study was to evaluate the significance of angiogenesis in laryngeal squamous cell carcinoma.

Patients and methods

A prospective study was conducted in the Otorhinolaryngology Department of Ain Shams University. Patients with laryngeal cancer, between 2006 and 2012, were enrolled. After surgery, histopathological examination with grading of tumours and immunostaining with CD34 antibody was carried out. Patients were followed up for 2 years and reassessment with immunostaining was carried out for recurrent tumours.

Results

Eighty cases were included. Results showed that tumours associated with a higher mean microvessel count had a highly significant association with nodal metastasis, recurrence and subsites, with poorer prognosis.

Conclusion

Results suggest that angiogenesis can be used as a prognostic factor in patients with laryngeal squamous cell carcinoma.

References

  1. Owen MR, Alarcón T, Maini PK, Byrne HM. Angiogenesis and vascular remodelling in normal and cancerous tissues. J Math Biol 2009; 58: 689–721.

    Article  Google Scholar 

  2. Sasano H, Suzuki T. Pathological evaluation of angiogenesis in human tumor. Biomed Pharmacother 2005; 59(Suppl 2):S334–S336.

    Article  CAS  Google Scholar 

  3. Hasan J, Byers R, Jayson GC. Intra-tumoural microvessel density in human solid tumours. Br J Cancer 2002; 86: 1566–1577.

    Article  CAS  Google Scholar 

  4. Kinoshita J, Kitamura K, Kabashima A, Saeki H, Tanaka S, Sugimachi K. Clinical significance of vascular endothelial growth factor-C (VEGF-C) in breast cancer. Breast Cancer Res Treat 2001; 66: 159–164.

    Article  CAS  Google Scholar 

  5. Nishida N, Yano H, Komai K, Nishida T, Kamura T, Kojiro M. Vascular endothelial growth factor C and vascular endothelial growth factor receptor 2 are related closely to he prognosis of patients with ovarian carcinoma. Cancer 2004; 101: 1364–1374.

    Article  CAS  Google Scholar 

  6. Amioka T, Kitadai Y, Tanaka S, Haruma K, Yoshihara M, Yasui W, Chayama K. Vascular endothelial growth factor-C expression predicts lymph node metastasis of human gastric carcinomas invading the submucosa. Eur J Cancer 2002; 38: 1413–1419.

    Article  CAS  Google Scholar 

  7. O-charoenrat P, Rhys-Evans P, Eccles SA. Expression of vascular endothelial growth factor family members in head and neck squamous cell carcinoma correlates with ymph node metastasis. Cancer 2001; 92: 556–568.

    Article  CAS  Google Scholar 

  8. Sullu Y, Gun S, Atmaca S, Karagoz F, Kandemir B. Poor prognostic clinicopathologic features correlate with VEGF expression but not with PTEN expression in squamous cell carcinoma of the larynx. Diagn Pathol 2010; 5: 35.

    Article  Google Scholar 

  9. Murray JD, Carlson GW, McLaughlin K, Pennington M, Lynn M, DeRose PB, et al. Tumor angiogenesis as a prognostic factor in laryngeal cancer. Am J Surg 1997; 174: 523–526.

    Article  CAS  Google Scholar 

  10. Burian M, Quint C, Neuchrist C. Angiogenic factors in laryngeal carcinomas: do they have prognostic relevance? Acta Otolaryngol 1999; 119: 289–292.

    Article  CAS  Google Scholar 

  11. Hagedorn HG, Nerlich AG. Microvessel density and endothelial basement membrane composition in laryngeal squamous cell carcinomas. Acta Otolaryngol 2000; 120: 891–898.

    Article  CAS  Google Scholar 

  12. Sion-Vardy N, Fliss DM, Prinsloo I, Shoham-Vardi I, Benharroch D. Neoangiogenesis in squamous cell carcinoma of the larynx — biological and prognostic associations. Pathol Res Pract 2001; 197: 1–5.

    Article  CAS  Google Scholar 

  13. Pietruszewska W, Kobos J, Gryczynski M. Prognostic significance of chosen tumour indicators (angiogenesis, cell proliferation and apoptosis) in laryngeal cancer. Int Cong Ser 2003; 1240: 1119–1123.

    Article  Google Scholar 

  14. Gryczynski M, Kobos J, Pietruszewska W. Intratumoral microvessels density and morphometric study of angiogenesis as prognostic factor in laryngeal cancer. Int Cong Ser 2003; 1240: 1113–1118.

    Article  Google Scholar 

  15. Rodrigo JP, Cabanillas R, Chiara MD, García Pedrero J, Astudillo A, Suárez Nieto C. Prognostic significance of angiogenesis in surgically treated supraglottic squamous cell carcinomas of the larynx. Acta Otorhinolaryngol Esp 2009; 60: 272–277.

    Article  Google Scholar 

  16. Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis — correlation in invasive breast carcinoma. N Engl J Med 1991; 324: 1–8.

    Article  CAS  Google Scholar 

  17. Shpitzer T, Chaimoff M, Gal R, Stern Y, Feinmesser R, Segal K. Tumor angiogenesis as a prognostic factor in early oral tongue cancer. Arch Otolaryngol Head Neck Surg 1996; 122: 865–868.

    Article  CAS  Google Scholar 

  18. Pazouki S, Chisholm DM, Adi MM, Carmichael G, Farquharson M, Ogden GR, et al. The association between tumour progression and vascularity in the oral mucosa. J Pathol 1997; 183: 39–43.

    Article  CAS  Google Scholar 

  19. Li C, Shintani S, Terakado N, Klosek SK, Ishikawa T, Nakashiro K, Hamakawa H. Microvessel density and expression of vascular endothelial growth factor, basic fibroblast growth factor, and platelet-derived endothelial growth factor in oral squamous cell carcinomas. Int J Oral Maxillofac Surg 2005; 34: 559–565.

    Article  CAS  Google Scholar 

  20. Shang ZJ, Li ZB, Li JR. VEGF is up-regulated by hypoxic stimulation and related to tumour angiogenesis and severity of disease in oral squamous cell carcinoma: in vitro and in vivo studies. Int J Oral Maxillofac Surg 2006; 35: 533–538.

    Article  Google Scholar 

  21. Dray TG, Hardin NJ, Sofferman RA. Angiogenesis as a prognostic marker in early head and neck cancer. Ann Otol Rhinol Laryngol 1995; 104(Pt 1): 724–729.

    Article  CAS  Google Scholar 

  22. Janot F, Klijanienko J, Russo A, Mamet JP, de Braud F, El-Naggar AK, et al. Prognostic value of clinicopathological parameters in head and neck squamous cell carcinoma: a prospective analysis. Br J Cancer 1996; 73: 531–538.

    Article  CAS  Google Scholar 

  23. Do NY, Lim SC, Im TS. Expression of c-erbB receptors, MMPs and VEGF in squamous cell carcinoma of the head and neck. Oncol Rep 2004; 12: 229–237.

    CAS  PubMed  Google Scholar 

  24. Vlachtsis K, Nikolaou A, Markou K, Fountzilas G, Daniilidis I. Clinical and molecular prognostic factors in operable laryngeal cancer. Eur Arch Otorhinolaryngol 2005; 262: 890–898.

    Article  Google Scholar 

  25. Kupisz K, Chibowski D, Klatka J, Klonowski S, Stepulak A. Tumor angiogenesis in patients with laryngeal cancer. Eur Arch Otorhinolaryngol 1999; 256: 303–305.

    Article  CAS  Google Scholar 

  26. Pignataro L, Carboni N, Midolo V, Bertolini F, Buffa R, Cesana BM, et al. Clinical relevance of microvessel density in laryngeal squamous cell carcinomas. Int J Cancer 2001; 92: 666–670.

    Article  CAS  Google Scholar 

  27. Beatrice F, Cammarota R, Giordano C, Corrado S, Ragona R, Sartoris A, et al. Angiogenesis: prognostic significance in laryngeal cancer. Anticancer Res 1998; 18(6B):4737–4740.

    CAS  PubMed  Google Scholar 

  28. Tse G, Chan A, Yu K. Strong immunohistochemical expression of vascular endothelial growth factor predicts overall survival in head and neck squamous cell carcinoma. Ann Surg Oncol 2007; 14: 3558–3565.

    Article  Google Scholar 

  29. Lentsch EJ, Goudy S, Sosnowski J, Major S, Bumpous JM. Microvessel density in head and neck squamous cell carcinoma primary tumors and its correlation with clinical staging parameters. Laryngoscope 2006; 116: 397–400.

    Article  Google Scholar 

  30. Ahlgren J, Risberg B, Villman K, Bergh J. Angiogenesis in invasive breast carcinoma — a prospective study of tumour heterogeneity. Eur J Cancer 2002; 38: 64–69.

    Article  CAS  Google Scholar 

  31. Kyzas PA, Stefanou D, Agnantis NJ. COX-2 expression correlates with VEGF-C and lymph node metastases in patients with head and neck squamous cell carcinoma. Mod Pathol 2005; 18: 153–160.

    Article  CAS  Google Scholar 

  32. Hannen EJ, van der Laak JA, Manni JJ, Freihofer HP, Slootweg PJ, Koole R, de Wilde PC. Computer assisted analysis of the microvasculature in metastasized and nonmetastasized squamous cell carcinomas of the tongue. Head Neck 2002; 24: 643–650.

    Article  Google Scholar 

  33. Schimming R, Marmé D. Endoglin (CD105) expression in squamous cell carcinoma of the oral cavity. Head Neck 2002; 24: 151–156.

    Article  Google Scholar 

  34. Zvrko E, Mikic A, Vuckovic L, Djukic V, Knezevic M. Prognostic relevance of CD105-assessed microvessel density in laryngeal carcinoma. Otolaryngol Head Neck Surg 2009; 141: 478–483.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of ORL and Head and Neck Surgery, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Egypt

    Osama M. Ibrahim, Hassan A. El-Ebiary, Samia A. Fawaz, Mohamed M. El-Sharnouby MD & Ahmed G. Khafagy

  2. Department of Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Nedal A. Hegazy

  3. Department of ENT, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Mohamed M. El-Sharnouby MD

Authors
  1. Osama M. Ibrahim
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  2. Hassan A. El-Ebiary
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  3. Nedal A. Hegazy
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  4. Samia A. Fawaz
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  5. Mohamed M. El-Sharnouby MD
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  6. Ahmed G. Khafagy
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Corresponding author

Correspondence to Mohamed M. El-Sharnouby MD.

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None declared.

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Ibrahim, O.M., El-Ebiary, H.A., Hegazy, N.A. et al. Does angiogenesis have a prognostic value in squamous cell carcinoma of the larynx?. Egypt J Otolaryngol 31, 92–97 (2015). https://doi.org/10.4103/1012-5574.156090

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  • DOI: https://doi.org/10.4103/1012-5574.156090

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