Table 1 Promoter sequences of interest for methylation analyses of GBJ2, GBJ6, and SLC26A4 genes, the expected sequence after bisulfite modification, and nucleotide dispensing queue of PyroMark Q24 sequencer

GJB 2

 Sequence to analyze

5′-GGCGCCGCTGGTGCCGGCGAAGCCCCGC-3′

 Sequence expected after bisulfite modification

5′-GGYGTYGTTGGTGTYGGYGAAGTTTTYGT-3′

 Nucleotide dispense queue

TGTCAGTCGTGTAGTCAGTCGATGTTCG

GJB 6

 Sequence to analyze

5′-GTTCCCTCGAGGGCCTGAGCAGGCGCCCCACACCTGCACCCGT-3′

 Sequence expected after bisulfite modification

5′-GTTTTTTYGAGGGTTTGAGTAGGYGTTTTATATTTGTATTYGT-3′

 Nucleotide dispense queue

TGTTCGATGTGACGTCAGTCGATTAGTATGTATCG

SLC26A4

 Sequence to analyze

5′-CGCCCGGCCCGGGCTCCACTCCCGGGGAGGCCTCGAGGGTTGCGGA-3′

 Sequence expected after bisulfite modification

5′-YGTTYGGTTYGGGTTTTATTTTYGGGGAGGTTTYGAGGGTTGYGGA-3′

 Nucleotide dispense queue

GTCGTCGTCGTTAGTTCGGATGTTCGAGTGTCG