Fig. 2

Example pyrogram. Methylation percentages of GJB2 gene for patients (first line), negative control (second line), and positive control (third line) are shown. Blue zones indicate the positions of C to T nucleotide substitution. The zones of no C to T nucleotide substitution indicate the presence of methylation as in the positive control. These percentages indicate the presence of C nucleotides, i.e., the percentages of methylation