Fig. 1

Methylated CpG islands in the positive control of GJB2 gene. Methylations were detected in all five regions as expected, which is marked by the persistence of Thymine (T) presence instead of being converted to Cytosine (C) (red arrows). The upper table shows the layout of samples in the equipment. All three genes were run together: GBJ2 in row A, for GJB6 in row B and SLC26A4 in row C. Positive and negative controls for the bisulfite conversion are in columns 7 and 8. The middle section shows the sequencing results of the current run, and the bottom part shows the expected sequencing results. The small red rectangle frames show the positions where C to T substitutions occur. Nucleotides at these positions are shown in the middle graph